منابع مشابه
Gel Electrophoresis
Electrophoresis is the migration of charged particles or molecules in an electric field. This occurs when the substances are in aqueous solution. The speed of migration is dependent on the applied electric field strength and the charges of the molecules. Thus, differently charged molecules will form individual zones while they migrate. In order to keep diffusion of the zones to a minimum, elect...
متن کاملGel Electrophoresis
Electrophoresis is the main metod for separating large polyelectrolyte molecules, primarily used on DNA, in biochemistry and medicine research. An overwiev of the models of physical mechanisms that are behind this phenomena will be presented. The prominent role is taken by reptation mechanisms, by which large exible polyelectrolytes thread their way through the pores of the gel matrix. There a...
متن کاملGel Electrophoresis
The separation of polypeptides by electrophoresis allowed Arne Tiselius to describe protein fractions corresponding to albumin, -, -, and -globulins in serum with the Rrst published diagram of human serum protein electrophoresis in 1939. The number of fractions slowly expanded into electrophoretic subfractions identiRed as 1, 2, 1, 2, 1 and 2. The mobility characteristics of these fractions are...
متن کاملGel Electrophoresis in Capillary Electrophoresis
The discovery that antigen}antibody interaction could be produced not only in liquids, but also on gel media, such as agar or agarose gels, with the formation of insoluble immunoprecipitates, opened the door to the development of the gel diffusion techniques for immunoprecipitation analysis. The Rrst of these techniques, known as double diffusion, was introduced by Ouchterlony in 1948. In this ...
متن کاملAgarose gel electrophoresis.
MATERIALS 6x Gel-loading buffer Agarose solutions (please see Step 3) DNA samples DNA size standards Samples of DNAs of known size are typically generated by restriction enzyme digestion of a plasmid or bacteriophage DNA of known sequence. Alternatively, they are produced by ligating a monomer DNA fragment of known size into a ladder of polymeric forms. DNA staining solution For a discussion of...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2019
ISSN: 2041-1723
DOI: 10.1038/s41467-019-11438-9